Preparation and analysis of spermatocyte meiotic pachytene bivalents of pigs for gene mapping

Well-spread meiotic pachytene bivalents were obtained by using the prolonged hypotonic treatment combined with high chloroform Carnory's fixative solution from cells of the testes of domestic pigs. Comparison in the division index and length of pachytene bivalents with metaphase chromosomes showed that those of the former are 5 times higher and 3.42(1.87-5.98) times longer than those of the latter. Comparative studies on chromomere maps of bivalents and mitotic chromosomal G-bands were conducted by using the chromosome 12 as a example. Sex vesicle and various shapes of synaptic sex chromosomes have been observed. Two-color PRimed IN Situ (PRINS) labeling has been conducted successfully on pachytene bivalents of pigs.     

Suppression of angiotensin Ⅱ stimulated responses in aortic vascular smooth muscle cells of experimental cirrhotic rats

INTRODUCTIONLivercirrhosisisoftencomplicatedbyhyperdynamiccirculation,whichischaracterizedbyadecreaseinarterialbloodpressureandperipheralvascularresistanceandanincreaseincardiacoutputandsplanchnicbloodnow[1].Thesehaemodynamicdisturbancesareproposedtocontributetothedevelopmelltofportalhypertension,retentionofsodiumandwater,ascitesformationandcorrelatewithprognosisoflivercirrhosis[2].Ithasbeensuggestedthatprimaryarterialvasodilationmayinducethishyperdynamiccirculation[3].Buttheunderlyingmec…     

Deoxyribozymes inhibit the expression ofperiod1 genein vitro

Throughout biology, a broad range of biochemical and physiological processes oscillate with approxi-mately 24-h rhythms or circadian rhythms as synchro-nizing with the rhythmic environment (day/night cy-cles, seasons, etc.). The circadian rhythms are under the control of an endogenous oscillator, the circadian clock[1]. Period (per), the first genetically identified circadian mutant[2], is assumed to be a key molecule in the regulation and functioning of the mammalian cir-cadian clock which is…     

红花玉兰等5个玉兰种花粉形态观察及分类学意义

通过对红花玉兰（Magnolia wufengensis L. Y. Ma et L. R. Wang）等5个玉兰种的花粉的光学显微镜和扫描电子显微镜观察拍照,测量光学显微镜下的花粉大小并对其进行统计学分析。结果表明：光学显微镜下,木兰属5个种的花粉均呈现椭圆形或近圆形,各种之间大小、形状方面有较大差异,种内部也存在不同程度的差异;扫描电子显微镜下,各种花粉均为舟形,且具有闭合、长及两端的远极萌发单沟等一致特征,花粉表面纹饰、突起等特征在种内部比较稳定,不同种之间差异明显,可以为木兰属植物种的划分提供比较准确的依据。     

LATE JURASSIC PROTOSUCHIAN SICHUANOSUCHUS HUIDONGENSIS (ARCHOSAURIA: CROCODYLIFORMES) FROM ZIGONG, SICHUAN, CHINA

AddihonaldiagnodsInternasialbarformedbypreInakilla;afaintmtCreSondorsalsurfaceoffrontalalongposterodorsalcomeroforbUpostorbitalcoInPleteyex-dudedfr0minfrateInporalfenestra;lengthofSymhysisbothanitSwidth;ventraledgeofangularexpandedtransverSeytoforml0ngitUdinalmwhalandlateralridges;denhckiinhpedgeofcheekteethlagrthanthoseinanteriorandposterioredges'RrmarksClosephylCgeneticrelahonshipsofShuhaandAstUngOSUchusfromtheInteJurassicandEariyCretaanusofthenorthernChinaareindicatalbythefollowing…     

小麦外源DNA导入及转化的初步研究

采用改进的酚-RNA酶法从野生一粒、野生二粒、提莫菲维和波兰小麦幼苗中提取总DNA,用子房注射法和花粉管途径导入云南铁壳麦1号和湖北生产上应用的鄂恩1号小麦,发现D1代出现供体性状或新的变异性状,有些变异性能遗传到后代。     

几种异质小麦核型的比较研究

本文以普通小麦中国春为对照,对(节节麦)中国春、(卵圆山羊草)中国春和(无芒山羊草)中国春3种同核异质杂种小麦的核型进行了分析比较,结果表明:它们的核型都与对照的一致,其核型公式均为2n=6x=42=36m+6sm,均属1A核型,从而证明了异源细胞质对普通小麦核型无明显影响。     

The cloning and preliminarily functional analysis of the human neurotrimin gene

~~The cloning and preliminarily functional analysis of the human neurotrimin gene~~